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KMID : 0545119910010020085
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Journal of Microbiology and Biotechnology
1991 Volume.1 No. 2 p.85 ~ p.89
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Purification and Characterization of Proteinaceous β-Lactamase Inhibitor from the Culture Broth of Streptomyces sp. SMF-19
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Kim, Myung Kuk
Kang, Hee Il/Lee, Kye Joon
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Abstract
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The aim of this study is to elucidate characteristics of β-lactamase inhibitor produced by Streptomyces sp. SMF-19 isolated from soil was found to produce proteinaceous extracellular β-lactamase inhibitor. The β-lactamase inhibitor was purified through ammonium sulfate fractionation, gel filtration, anion exchange chromatography and fast performace liquid chromatography. The molecular weight of the β-lactamase inhibitor was estimated to be about 48,000 by SDS-PAGE. The mode of inhibition against penicillin G as a substrate was uncompetitive. The β-lactamase inhibitor was stable in wide pH range but unstable at high temperature above 50℃.
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